DNA Next Generation Sequencing (NGS) refers to a group of advanced sequencing technologies used to rapidly sequence DNA and RNA, allowing for high-throughput, large-scale genomic analysis. NGS has revolutionized the field of genomics by enabling large-scale sequencing projects at a fraction of the cost and time compared to traditional Sanger sequencing.
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๐๐ฉ๐ฉ๐ฅ๐ข๐๐๐ญ๐ข๐จ๐ง๐ฌ ๐จ๐ ๐๐๐:
- Whole Genome Sequencing (WGS): Sequencing the entire genome of an organism, providing a comprehensive understanding of genetic variation.
- Exome Sequencing: Focuses on sequencing the protein-coding regions of the genome (the exons), which represent only about 1-2% of the total genome but contain the majority of disease-causing mutations.
- RNA Sequencing (RNA-Seq): Used to analyze the transcriptome, or the set of RNA molecules expressed in a given tissue or cell type, helping to understand gene expression patterns.
- Metagenomics: Used to sequence DNA from complex environmental samples (e.g., soil, water, or the human microbiome) to identify and characterize microbial communities.
- Targeted Sequencing: Focuses on specific genomic regions, such as known mutations associated with diseases or specific genes of interest.
- Cancer Genomics: NGS is widely used to detect mutations in cancer-related genes, enabling personalized treatments based on the genetic profile of a patientโs tumor.
๐๐ก๐ ๐๐ข๐ฌ๐ญ ๐จ๐ ๐๐จ๐ฆ๐ฉ๐๐ง๐ข๐๐ฌ:
- Illumina, Inc.
- Thermo Fisher
- Qiagen N.V.
- Beijing Genomics Institute
- PerkinElmer, Inc.
- Hoffman-La Roche Ltd.
- Agilent Technologies
- Eurofins Scientific
- Oxford Nanopore Technologies Ltd.
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